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Thermo Fisher
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Image Search Results
Journal: Molecular Cell
Article Title: Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans
doi: 10.1016/j.molcel.2020.10.012
Figure Lengend Snippet:
Article Snippet: Chimerism in the bone marrow was determined after red cell lysis using a cocktail containing antibodies against lineage antigens (FITC-conjugated, as above),
Techniques: Recombinant, Sequencing, Clone Assay, Software
Journal: International Journal of Nanomedicine
Article Title: Greater Plasma Protein Adsorption on Mesoporous Silica Nanoparticles Aggravates Atopic Dermatitis
doi: 10.2147/IJN.S383324
Figure Lengend Snippet: Higher T cell immune responses to MSN in AD skin inflammation. Serum total IgE, DFE-specific IgE ( A ), and serum total IgG2a ( B ) levels were measured using ELISA. Blood samples of various groups, the vehicle (Ctrl), DFE plus the vehicle, and DFE plus nanoparticles, were collected by an orbital puncture on day 21. ( C ) CD4 + T cells in LNs of mice treated with CSN-Corona or MSN-Corona on day 21 after AD induction were analyzed by intracellular cytokine staining for FACS analysis. Numbers in quadrants indicate the percentages of cells expressing CD4 + IFN-γ, IL-4, or IL-17A. Statistical analysis of IFN-γ-, IL-4-, or IL-17-expressing CD4 + T cells in the LNs. ( D ) Expression of cytokines in the ears of CSN-Corona- or MSN-Corona-treated AD mice. To determine the cytokine levels in mice, the ears were excised on day 21. Gene expression was analyzed using qPCR. The gene expression levels were normalized to the β-actin level, and the values of fold-changes are presented. ( E ) Schematic illustration of T cell-mediated immune reactions from AD skin tissues upon MSN and CSN treatment. The data are presented as the mean ± SD of five determinations. * P < 0.05.
Article Snippet: Subsequently, the cells were stained using mouse CD4 PerCP-Cy™5.5-FITC-conjugated Th1 (IFN-γ), CD4 PerCP-Cy™5.5-APC-conjugated Th2 (IL-4), and
Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing